باکتری های تجزیه کننده تانن ها و ترکیبات فنلی در شکمبه

Author links open overlay panelSrobana Sarkar a 1, Pankaj Kumar Kumawat a 1, Randhir Singh Bhatt a, Artabandhu Sahoo a bShow moreAdd to MendeleyShareCitehttps://doi.org/10.1016/j.smallrumres.2024.107272Get rights and contentHighlights•Tainolytic bacteria were isolated from rumen contents of Malpura sheep.•Out of 24 isolates, 16 belonged to order Lactobacillales with taase activity ranging from 1.2-12.5 mol gallic acid/min.•Total cellulolytic enzyme activity (FPase) of the isolates ranged from 0.14 to 9.80 U/mL.•Isolated tainolytic strains effectively degraded Prosopsis cineraria leaves showing potential to be used as direct-fed microbials in ruminants for utilization of tain-rich fibrous feeds.AbstractTo counteract the negative effects of phytotains, ruminants host a diverse community of bacteria that break down tains. These bacteria not only possess tainolytic activity but also have other functional relevance that hasn't been properly examined. Therefore, the present study was conducted to isolate and characterize tain degrading bacteria (TDB) from rumen of sheep fed tain-rich diet with possible fibrolytic activity. Based on the development of a clear zone on tain-enriched agar plates, a total of 24 isolates were screened as tain hydrolyzing bacteria. Majority of the isolates were gram positive cocci. The isolates could tolerate phenolic monomers such as ferulic acid, gallic acid, vanillic acid up to 30 mM and pyrogallol up to 10 mM, while failed to gro